Beilstein J. Nanotechnol.2018,9, 850–860, doi:10.3762/bjnano.9.79
as individual cells at subcellular resolution.
Keywords: double-barrelpipette; hydrodynamic confinement; perfusion; oxygen flux; single-cell metabolic analysis; Introduction
Transport, production and consumption of gasses, ions, and organic molecules are fluxes that sustain life. Relatively few
studied below using a finite element model (FEM) of the double-barrelpipette with perfusion. To validate this model, we first compare hydrodynamic confinement obtained from the model with experiments using a fluorescent dye. Later in the paper, we also show that FEM results agree qualitatively with a
outside the channel, unless the flow velocity is high enough that the molecule moves through this section before it has a chance to diffuse through the gap. Therefore, in the section where the channel walls are missing, like the section at the tip of the double-barrelpipette, the molecule could remain
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Figure 1:
a) Illustration of the double-barrel perfusion-based single-cell respirometry probe. The cell cultu...